脂肪乳剂对完全胃肠外营养大鼠血清游离脂肪酸谱的影响

THE EFFECT OF FAT EMULSIOS ON SERUM FATTY ACID PROFILES IN TPN RATS

为研究脂肪乳剂对机体脂肪代谢的影响，用气相色谱法观测了完全胃肠外营养（ＴＰＮ）大鼠血清游离脂肪酸（ＦＦＡ）谱的变化。将４０只雄性Ｗｉｓｔａｒ大鼠随机分成５组，每组８只：（１）ＳＨ（Ｓｈａｍｏｐｅｒａｔｉｏｎ）组，仅进行颈外静脉结扎，正常饲养；（２）ＮＳ（Ｎｏｔｍａｌｓａｌｉｎｅ）组：正常饲养，输入生理盐水；（３）ＧＳ（Ｇｌｕｃｏｓｅ）组：全部能量由葡萄糖提供，即不含脂肪乳剂ＴＰＮ组；（４）ＬＣＴ（Ｌｏｎｇｃｈａｉｎｔｒｉｇｌｙｃｅｒｉｄｅ）组：含１０％Ｉｎｔｒａｌｉｐｉｄ脂肪轧的ＴＰＮ组；（５）ＭＣＴ（Ｍｅｄｉｕｍｃｈａｉｎｔｒｉｇｌｙｃｅｒｉｄｅ）组：含１０％Ｌｉｐｏｆｕｎｄｉｎ脂肪乳ＴＰＮ组。ＴＰＮ各组大鼠等能量等氮量等液量匀速连续输入“生合一”营养液（即将所有营养物混合在一个溶器内）。于第７天取血标本进行血清ＦＦＡ测定。结果表明：不含脂肪乳的ＴＰＮ大鼠血清各种ＦＦＡ下降，并出现必需脂肪酸缺乏（ＥＦＡＤ）症状，如精神不振活动少，毛发无光泽、脱落等；脂肪乳剂的使用，使大鼠血清各种ＦＦＡ升高，对机体的代谢和功能有着不同的生理、药理作用。

at emulsion used in total parenteral nutrition(TPN)has become acommon clinical practice,To investigate the effect of intravenous fat emul-sions on fat metabolism,the serum free fatty acid(FFA)profiles ofTPN rats receiving either 10%Intralipid(LCT)or 10%Lipofundin(MCT/LCT)were studied byGC-MS.Forty Wistar male rats were divided randomly into 5 groups,8 rats iueach group.(1) Group SH (Control),was fed with regular chow;(2)Gr-oup NS (Control),with saline infusion and regular chow;(3)Group GS,with fat-free TPN;(4) Group LCT,with 10%Intralipid (LCT)as a partial calories for TPN;(5)Group MCT,with 10%Lipofundin (MCT/LCT)as a partial calories for TPN. All TPN-group rats were continuously givenisocaloric, isonitrogenous and isovolemic“All-in-One”solutions. Serumsamples were drawn on the 7th day after TPN for fatty acid analysis andbiochemical measurements.The results suggested that:(1)hFAD was appeared in fat-free TPNrats.(2) Fat emulsion infusion induced a high increase in serum FFA inTPN rats,along with the elevated availability of EFA. It might havephysiological and pharmacological effects on body metabolism and func-tion.