不同剂量维生素C对C_（57）小鼠Lewis肺癌移植瘤的形成及转移的影响

THE EFFECT OF DIFFERENT DOSES OF VITAMIN C ON TUMOR FORMATION AND METASTASIS OF TRANS-PLANTED LEWIS LUNG CARCINOMA IN C_(57) MICE

用Ｃ＿（５７）小鼠腋下接种Ｌｅｗｉｓ肺癌细胞，每天分别灌胃，补充不同剂量维生素Ｃ，研究不同剂量维生素Ｃ对Ｌｅｗｉｓ肺癌移植瘤的形成及转移的影响。结果表明：补充高剂量维生素Ｃ（０．２５ｍｇ·ｇｂｗ￣（－１）·ｄ￣（－１））对小鼠Ｌｅｗｉｓ肺癌细胞皮下移植瘤的形成及肺转移有明显的抑制作用，而补充低剂量维生素Ｃ（０．０５ｍｇ·ｇｂｗ￣（－１）·ｄ￣（－１））则未见明显作用。实验早期，高剂量维生素Ｃ组鼠肝脏内维生素Ｃ含量在三组中最高，可能与其抑瘤效果较好有关。低剂量维生素Ｃ组鼠血浆脂质过氧化物含量显著高于高剂量维生素Ｃ组，血红细胞内超氧化物歧化酶活性则有降低趋势，可能与其抑瘤效果不明显有关。补充高剂量维生素Ｃ并不影响实验鼠体重。

ne hundred and twenty two C57 BL inbred strain mice(male)were used to study the effect of different doses of vitamin C on tumor formation and rnetastasis of trallsplanted Lewis lung carcinoma(LLC).The resultsshowed that:supplementing high dose vitamin C (0.25mg·g bw-1·d-1)significantly inhibited subcutaneous transplanted tumor growth of LLC andthe metastases in the lung; but low dose vitamin C(0.05mg·gbw-1.d-1)had nosignificant effect.In he early period after supplementing vitamin C,theliver vitamin C level of high dose vitamin C group was significantlyhigher than that of the other groups: this might be interrelated withthe inhibiting effect of the high dose vitamin C on tumor growth.Theplasma LPO content of the low dose vitamin C group was higher than thatof the high dose vitamin C group, but the RBC SOD activity tended todescend.This might be one of the biochemical mechanisms to explain whythe low dose vitamin C had no significant inhibiting effect on tumorgrowth.Application of high dose vitamin C has not effected the bodyweight of the experimental mice. So the inhibiting tumor effect might notbe the result of the body weight decrease.