诱导根癌农杆菌vir区基因表达水稻代谢物的分离、鉴定和生物活性研究

The Purification, Indentification and Biological Activity of metabolites Capable of Inducing Agrobacterium vir gene Expression Isolated from Rice (Oryza sative L.)

从孕穗期至开花期水稻(oryza satire L.cv.India IR 72)的茎叶提取液中,筛选并分离出二种能高效诱导根癌农杆菌(Agrobacterium tumfaciens)vir区基因表达的信号分子,这两种信号分子的化学结构分别是5,7,4′-三羟基-3′-5′-二甲氧基黄酮和5,4′-二羟基-3′,5′-二甲氧基-7-β-D-葡萄糖氧基黄酮。

Two flavones are isclated from leaf and stem extracts of panicle-differentiating to flowering rice (Oryza sative L.) plants. Based on the spectroscopy analysis, the structures of these two compounds are identified as 5, 7, 4'-trihydroxy-3',5'-dimethoxy-flavone (A) and 5, 4' -dihydroxy-3', 5'-dimethoxy-7-β-D-glucosyloxy-flavone (B), respectively. These two compounds were able to strongly induce vir gene expression of Agrobacterium tumefaciens.