应用RAPD技术评价丁香品种间遗传关系

Analysis of Genetic Relationship among Lilacs(Syringa)by RAPD

采用随机扩增多态ＤＮＡ（ＲＡＰＤ）技术分析了６个丁香（Ｓａｒｉｎａａ）品种间的遗传关系。用１７个单引物共扩增出８３个ＤＮＡ片段，其中清晰可辨、重复的片段６２个，占总数的７４．６％。被检测出品种特有标记，可作为区别不同品种的重要性状。品种ｆｌｏｒａ和ＮａｕｄＮｏｔｃｕｔｔ间相似系数高达９８．１％，证明它们是同一品种不同无性系。通过品种间相似系数比较，确认品种Ｒｏｃｈｅｓｔｅｒ是从品种Ｆｌｏｒａ选育而来；品种Ｍａｉｄｅｎ’ｓＢｌｕｓｈ是由朝鲜丁香（Ｓｙｒｉｎｇａｏｂｉａｔａｖａｒ．ｄｉｌａｔａｔａ）和欧丁香品种ＭｒｓＭａｒｒｙＢｉｃｋｌｅ杂交而来。

Genetic relationship among lilac(Syringa)cultivars were analysed using random amplified polymorphic DNA(RAPD)with an average of 5 bands each primer.Among them,clear and reproducible bands were 62,accounting for74.6%.Cultivar specific bands of lilacs were also scored,which were important characteristies for distinguishing lilac cultivars.The similarity between Flora and Naud Notcutt was 98.1%,which demonstrated that they were different clones under the same cultivar.Compared with similarities among cultivars,Syringa vulgaris cv.Rochester would be selected from' Florall',and S. x hyacinthiflora cv.Maiden's Blush could be from hybridization between S. oblata var.dilatata and S.viugaris cv.Mrs Marry Bickle.RAPD technique is a valuable and reliable method to identify and classify lilac cultivars and other horticultural germplasm as well.