大白菜和紫菜薹自交系染色体组DNA的RAPD分析

RAPD Markers of Genomic DNA in Chinese Cabbage and Purple Cai-tai Inbred Lines

用８个随机引物（１０ｂｐ）对２个紫菜薹自交系的３个单株和４个大白菜自交系的４个单株的染色体组ＤＮＡ进行ＰＣＲ扩增，共有４０条带分离清晰、明亮，其中引条带在７个单株中表现出差异，可作为遗传标记（ＲＡＰＤ标记）。４个大白菜自交系之间的平均差异条带数为１２．３，２个紫菜薹自交系之间为９．５，大白菜与紫菜薹之间平均有１５．５条带的差异。用７个引物在９３Ｗ０５－７（紫菜薹）和９３Ｗ５８－５（大白菜）之间检测出对个ＲＡＰＤ标记。从同一自交系的不同单株抽提的染色体组ＤＮＡ扩增出较一致的ＤＮＡ谱带，而不同自交系间扩增出的ＤＮＡ谱带差异很大，这表明此方法也可以象ＲＦＬＰ一样，用于大白菜和紫菜薹的分子标记。

The genomic DNA isolated from four inbred lines of Chinese cabbage (Brassica campestris L. ssp. pekinenese) and two inbred lines of purple cai-tai(B.campestris L. var. purpurea) was used in this study to generate the RAPD markers by eight arbitrary 10-mer primers. The amplified fragment size ranged from 300 to 2500 bp. Fourty fragments showed clear and robust bands on the gel. Among them, 31 bands amplified by 7 primers provided polymorphism between 6 inbred lines, and could be used as molecular markers. By using 7 primers, it assayed 21 RAPD markers between 93W05-7 (purple cai-tai) and 94W58-5 (Chinese cabbage). The average number of polymorphic markers between Chinese cabbage and purple cai-tat was 15.5, but among them was 12.3 and 9.5respectively. The RAPDs of two individual plants from one inbred lines were very similiar.RAPD markers like RFLP markers could also be used as molecular markers in Brassica.