摘要
人乙型肝炎病毒（ａｄｒ亚型）表面抗原Ｓ基因克隆到ＰｕＡＣ－５苜蓿尺蠖核型多角体病毒转移载体上，所构建的重组转移载体质粒与野生型ＡｃＮＰＶＤＮＡ共转染Ｓｆ－２１细胞，经空斑法筛选和纯化，得到了含ＨＢＶＳ基因的重组病毒ＡｃＮＰＶＳ．用放射免疫法测定了ＡｃＮＰＶＳ感染的Ｓｆ－２１细胞及其培养液中的ＨＢｓＡｇ，总表达量为１．２２μｇ／１０￣６细胞．
The S gene sequence from HBV(adr sub-type)was inserted into a Autographa cali-fornica nuclear polyhedrosis virus(AcNPV ) vector PuAC 5 to construct a recombinant transfervector plasmid PuACHS-5. The Sf-21 cells were co-transfected with PuACHS-5 DNA and wild-type Ac NPV DNA.Arecombinant virus AcNPVS was selected and purified by plaque assay. Thelevel of HBsAg expression in Sf-21 ceIls infected with recombinant virus AcNPVS was estimated atatotal of l.22 t1gl106 cells by radioimmunoassay.
关键词
乙型肝炎病毒
表面抗原
昆虫细胞
基因表达
Autographa californica nuclear polyhedros is virus
hepatitis B virus surface anti-gen
insect cell
gene expression
