一串红花叶病及其病原研究

Studyon on the pathongens causing mosaic disease of Aalvia splandens

１９８９─１９９２年间对杭州等地一串红（Ｓａｌｖｉａｓｐｌｅｎｄｅｎｓ）花叶病的发生及其病原进行了系统研究，从病株上分离得到的１７个病毒分离物，经生物学和血清学反应测定均符合黄瓜花叶病毒（ＣＭＶ）的特点。病毒分离物Ｍ－２２不能经由桃蚜传播使黄苗榆等供试寄主发病。用０．５ｍｏｌ／Ｌ磷酸钾盐缓冲液对Ｍ－２２进行提纯，提纯病毒经醋酸铀负染，测定其粒子大小为３０．２ｎｍ。ＳＤＳ－聚丙烯酰胺凝胶电泳测定Ｍ－２２的外壳蛋白分子量约为２８ｋＤ，经琼脂双扩散测定，Ｍ－２２与几个已知ＣＭＶ抗血清均有强阳性反应，Ｍ－２２与一个番茄不孕病毒（ＴＡＶ）抗血清有阳性反应，但其抗血清与供试ＴＡＶ抗原无阳性反应。ＥＬＩｓＡ反应测定结果表明，Ｍ－２２与一个来自于十字花科大白菜上的ＣＭＶ分离物和一个来自于前科烟草上的ＣＭＶ分离物，在血清学关系上有明显差异。根据实验结果，作者认为目前在杭州等地侵染一串红的病原病毒主要是ＣＭＶ，侵染一串红的ＣＭＶ分离物Ｍ－２２可能是一个独立的血清型．

Seventeen virus isolates were obtained from diseased plants of Salvia splendens.All the isolates were identified as cucumber mosaic virus(CMV)on the basis of biological properties,morphological and serological characteristics. One tested isolate M-22 was not transmitted by Myzus persicae in non-persistant manner. A modified schedule for purification was applied whichyielded virus 10 mg/100 g fresh tobacco leaf.One single protein species that was about 28. 0 kDwas detected by SDS polyacrylamide gel electrophoresis。When tested by gel-diffusing and ELISA,the isolate differed serologically from two CMV isolates from Brasica napus(CMV-H2)and tobac-co(CMV-8)respectively,This isolate as showed weak reaction with an antiserum of tomato as-permy virus.The isolates from S. splendens was considered as a separate serologicalgroup ofCMV.CMV was considered as the main pathogen causing mosaic disease on Salvia splendens.