稻田中硫酸盐还原菌的数量和优势种群

Amounts and dominant species of sulfate-reducing bacteria(SRB)in submerged paddy soils with different fertilizers.

采用改良的Ｈｕｎｇａｔｅ厌氧操作技术，研究分析了水稻田中硫酸盐还原细菌（ＳＲＢ）的数量和优势种。结果表明，早稻在分蘖盛期至分蘖末期行株间土壤中的ＳＲＢ数量达１０￣７个／克于土，到孕穗末期减少２个数量级。施腐熟牛粪的水稻行株间土壤中的ＳＲＢ数量略高。施腐熟牛粪同施ＫＣ１和菜籽饼比较，施腐熟牛粪对ＳＲＢ的生长有一定的刺激作用。在稻田土壤中的ＳＲＢ数量以５—１３ｃｍ土层为多，水稻根系土壤中的ＳＲＢ数量明显高于行株间土壤中的ＳＲＢ数量。在乳酸钠、乙酸钠、Ｎ＿２／ＣＯ＿２及混合基质中生长的ＳＲＢ数量无明显差异。经分离纯化到的ＳＲＢ优势菌株，经初步鉴定可为Ｄｅｓｕｌｆｏｖｉｂｒｉｏ的一个种和Ｄｅｓｕｌｆｏｔｏｍａｃｕｌｕｍ的一个种。

With improved Hungate anaerobic technique,the amounts and species of sulfate-re-ducing bacteria(SRB)in the submerged paddy soil planted rice and used different fertilizers werestudied.The results obtained showed that application of cow manure stimulated growth of SRB ascompared with that used KCl and cake fertilizer at a certain extent.Amount of SRB was highergenerally in 5-13 cm depth of soil than that in 0-5cm and 13-18 cm depth of soil.The amountsof SRB were higher in root system soils than that in interrow soils.The resuIt also showed thatthere was no marked difference among amounts of SRB grown on lactate,acetate,H_2/CO_2 andmixed substrates. The dominant species of SRB in the paddy soil were Desulfovibrio sp.and Desul-fotomaculum sp.