速冻中低温保护剂浓度对小鼠胚胎发育的影响

Study of t he effects of cryoprotectant concentrations on mouse embryo development with rapidfreezing method.

用快速冷冻方法，冷冻小鼠的２－细胞胚、４－细胞胚、８－细胞胚、桑椹胚和囊胚，低温保护液分别含有０．５，１．５，２．５，４ｍｏｌ／Ｌ丙二醇，含１０％的犊牛血清和０．２５ｍｏｌ／Ｌ蔗糖，对于８－细胞以前的小鼠胚胎，快速冷冻保存时丙二醇的浓度在２．５ｍｏｌ／Ｌ和４ｍｏｌ／Ｌ时效果较好，随着小鼠早期胚胎（囊胚以前）的发育，抗低温冷冻的能力加强。

cell,4-cell,8-cell embryos,morula and blastocyst of mice were frozen in differentconcentrations of propylene glycol with rapid freezing method.When the mouse embryos before 8-cell were frozen , it is better to have the glycerol concentrations more than 2.5 mol/L.The em-bryos got higher developing rate frozen with 4 mol/L propyleng glycol.The abilities of enduringlow temperature for mouse embryos were enhanced with embryo development.