PP_（333）对马铃薯试管苗生长和长期保存的影响

The effects of paclobutrazol on in vitro growth and long-term preservation of potato seedlings

研究了自然温度与恒温培养条件下，不同ＰＰ_（３３３）浓度处理对马铃薯试管苗生长和长期保存的影响，结果表明，自然温度培养对马铃薯试管苗延缓生长、延长保存时间、提高存活率更有利，存活率可达１００％，还有促进试管苗结薯的作用。随ＰＰ_（３３３）使用浓度的增加，抑制试管苗生长的作用也增强，保存时间也相应延长。１０ｍｇ／Ｌ处理经３００天培养后，株高几乎与原接种时一样，经ＰＰ_（３３３）处理的试管苗比对照矮壮、叶数增多、根数少而粗短，存活率高。试管苗继代保存期由１年转管４－５次，延长到１－１．５年转管１次。当转入恢复生长的培养基１０天后，就能恢复至正常生长状态。

The experiment was conducted to study the effects of different concentrations of PP_(333) on in vitro growth and long-term preservation of potato (foreign No. 2)seedlings under natural or constant temperature conditions. Compared with constant temperature,it was favourable for retard-ing seedlings growth, prolonging in vitro preservation duration and increasing survival rate up to 100%, In addition,it showed the effect of acceleration on in vitro tuberization,with the increasing concentrations of PP_(333),the inhibition of in vitro growth became more intensive and the prolonga-tion of in vitro preservation was evidenced accordingly,the height of plants after 300 days' culture,treated with 10 mg/L PP_(333) was almost the same as that before treatment,treated with PP_(333)seedlings had more leaves, less roots, stout shape and high survival rate,which reduced the trans-ferring times from 4-5per year to 1 per 1-1.5 year. After transferred to culture medinum for re-covery growth,it could be quickly tured back to normal growth after 10 days.