栽培稻和野生稻杂种花药培养效率的研究

Study on efficiency of anther culture in interspecific hybrids between cultivated rice and O. rufipogon:

对１３份栽培稻与普通野生稻（Ｏ．ｒｕｆｉｐｏｇｏｎ）种间杂种的花药培养效率进行了研究。结果表明，栽野稻杂种间花培效率存在明显差异，愈伤组织诱导率平均１４％，变幅０５～３５％；绿苗分化率平均２２０％，变幅０～５９７％；亲本影响杂种的花培力。改良Ｎ６培养基可显著提高栽培稻和野生稻杂种的花培效率，且优于其他４种供试培养基。

Thirteen interspecific hybrids between cultivated rice and O. rufipogon have been studied on the efficiency of anther culture. The result indicated that a abvious difference in efficiency of anther culture existed among the interspecific hybrids tested, in which the rates of the callus induction and the green plantlet differentiation were 1 4% and 22 0% in everage, ranged in 0 5～3 5% and 0～59 7%, respectively. Parent could influence anther culture ability of their hybrids. It has been showed that the improved N6 medium is better than other 4 media tested, which apparently increase in efficiency of anther culture of the interspecific hybrids.