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六棱大麦“Frances”游离小孢子的分离、培养与植株再生 被引量:1

In-vitro culture of isolated microspores and plant regeneration in six-row barley (Hordeum vulgare L.) cv.Frances
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摘要 大麦小孢子单核中期的穗,经4℃预处理4星期后,直接剪成小段,捣碎、过滤、离心和洗涤后收集小孢子。用MSm+Maltose60.0g/L+1.0mg/LBAP对小孢子慢速振荡培养,7天后可观察到小孢子分裂的小细胞团,经3星期左右培养,小细胞团总数达最高,其频率为总小孢子的2.36%。这些小细胞团80%发育为类似体细胞胚结构,20%为典型愈伤组织,类似体细胞结构胚置于1/2MS+1.0mg/LBAP+0.2mg/LNAA+0.2mg/LZT分化培养基上,总分化频率为67%,其中绿苗率为39%,愈伤组织经胚性改造后,在1/2MS+4.0mg/LBAP+0.5mg/LNAA+0.5mg/LIAA培养基上分化,总分化频率为62%,绿苗率为18%。 Microspores were isolated from the spike of the six-row barley (Hordeum vulgare L,) cultivarFrances at the mid-unicellular pollen stage, pretreated at 4℃ for 4 weeks, by directly cutting the spike intosmall segments and homogenizing. The isolated microspores were incubated in MSm medium supplementedwith 60 g/ L maltose, 1.0mg/ L BAP with slowly shaking at 25℃ in darkness. Cell aggregatesfrom microspores were observed after 7 days from in initial culturing and the number of them reached tothe highest point at a frequency of 2.36% after about 3 weeks of culture. The cell aggregates developedembryo-like structure and call us at the rate of 80% and 20%, respectively. The embryo-like structureson 1/2 MS containing 1.0mg/ L BAP, 0.2mg/ L NAA and 0.2mg/ L ZT regenerated green plantlets(39%) and albino plantlets (28%). The calli by being improved on L3 medium with 2.0 mg / L 2, 4-D,0.1mg / L ABA and 0.2mg / L ZT, regenerated green plantlets (18%) and albino plantlets (44%), on 1 / 2MS medium by adding 4.0 mg / L BAP, 0.5mg / L N AA and 0.5mg/ L 1AA.
作者 项友斌 刘静
出处 《浙江农业学报》 CSCD 1995年第3期182-186,共5页 Acta Agriculturae Zhejiangensis
关键词 六棱大麦 游离小孢子 细胞团 植株再生 大麦 Six-row barley (Hordeum vulgare L.) Isolated microspore Cell aggregate, Plant regeneration
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  • 1A. Ziauddin,A. Marsolais,E. Simion,K. J. Kasha. Improved plant regeneration from wheat anther and barley microspore culture using phenylacetic acid (PAA)[J] 1992,Plant Cell Reports(10):489~498
  • 2A. Ziauddin,E. Simion,K. J. Kasha. Improved plant regeneration from shed microspore culture in barley (Hordeum vulgare L.) cv. igri[J] 1990,Plant Cell Reports(2):69~72
  • 3Marc Paul Coumans,Sukhinder Sohota,Eric Bruce Swanson. Plant development from isolated microspores of Zea mays L.[J] 1989,Plant Cell Reports(8):618~621
  • 4Z. M. Wei,M. Kyo,H. Harada. Callus formation and plant regeneration through direct culture of isolated pollen of Hordeum vulgare cv. ‘Sabarlis’[J] 1986,Theoretical and Applied Genetics(2):252~255
  • 5Z. M. Wei. Pollen callus culture in Triticum aestivum[J] 1982,Theoretical and Applied Genetics(1):71~73
  • 6Gerhard Wenzel,Franz Hoffmann,Ingo Potrykus,Emrys Thomas. The separation of viable rye microspores from mixed populations and their development in culture[J] 1975,MGG Molecular & General Genetics(4):293~297

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