聚合酶链反应检测临床标本中的肺炎支原体

DETECTION OF MYCOPLASMA PNEUMONIA IN CLINICAL SAMPLES BY POLYMERASE CHAIN REACTION

作者应用聚合酶链反应（ＰＣＲ）检测３～１３岁肺炎患儿１５０例咽拭标本中的肺炎支原体（ＭＰ），以分离培养及间接血凝试验（ＩＨＡ）为对照。ＰＣＲ检出率显著高于培养，若以ＩＨＡ为标准，ＰＣＲ阴性一致率为９６％、阳性一致率为７３％。不同病程ＭＰ－ＤＮＡ检出率无显著差异，提示即使病程较长仍可应用ＰＣＲ作出诊断。鉴于ＰＣＲ检测简便快速，可作呼吸道ＭＰ感染的常规诊断应用。但ＭＰ感染经敏感药物治疗可使ＰＣＲ结果阴性，因此，血清学检测尚不宜放弃。

Polymerase chain reactian(PCR)was applied to the detection of mycoplasma pnenmonia (MP) in throat-swab samples from 150 children with pneumonia aged from 3 to 13 years and was also compared with isolated culture and indirect haemagglutination assay(IHA)for MP.The positive rate of MP-PCR was much higher than that of the culture. According to the criterion of IHA, the positive coincident rate and negative coincident rate of MP-PCR were 73% and 96% respectively. There were no significant differences in MP-PCR-positive rates of these case with different durations, which means MP-PCR is also helpfuI to the diagnosis of patients with a long duration, It is concluded that PCR,as a rapid and simple method,is fit for routinly use on specimens from patients suspected to have MP infection ,but the serological test should not be given up because of the false negative results of MP-PCR in samples after effective antibiotics therapy.