摘要
作者应用聚合酶链反应(PCR)检测3~13岁肺炎患儿150例咽拭标本中的肺炎支原体(MP),以分离培养及间接血凝试验(IHA)为对照。PCR检出率显著高于培养,若以IHA为标准,PCR阴性一致率为96%、阳性一致率为73%。不同病程MP-DNA检出率无显著差异,提示即使病程较长仍可应用PCR作出诊断。鉴于PCR检测简便快速,可作呼吸道MP感染的常规诊断应用。但MP感染经敏感药物治疗可使PCR结果阴性,因此,血清学检测尚不宜放弃。
Polymerase chain reactian(PCR)was applied to the detection of mycoplasma pnenmonia (MP) in throat-swab samples from 150 children with pneumonia aged from 3 to 13 years and was also compared with isolated culture and indirect haemagglutination assay(IHA)for MP.The positive rate of MP-PCR was much higher than that of the culture. According to the criterion of IHA, the positive coincident rate and negative coincident rate of MP-PCR were 73% and 96% respectively. There were no significant differences in MP-PCR-positive rates of these case with different durations, which means MP-PCR is also helpfuI to the diagnosis of patients with a long duration, It is concluded that PCR,as a rapid and simple method,is fit for routinly use on specimens from patients suspected to have MP infection ,but the serological test should not be given up because of the false negative results of MP-PCR in samples after effective antibiotics therapy.
关键词
聚合酶链反应
诊断
肺炎支原体
肺炎
Polymerase chain reaction/diag use
Mycoplasma pneumonia
Pneumonia,mycoplasma/diag