灰树花多糖促进干扰素诱生作用的研究

ENHANCEMENT OF MOUSE INTERFERON INDUCTION IN VIVO BY GRIFOLAN OLYSACCHARIDE

灰树花多糖以２０００，１００Ｏ，５００ｍｇ／ｋｇ／ｄ的剂量给ＮＩＨ小鼠灌胄７天，然后腹腔注射１２８０ＨＡＵ／ｍｌ的ＮＤＶ（新城鸡瘟病毒）０．５ｍｌ。６ｈ后放血，分离血清，以Ｌ９２９细胞、ＶＳＶ病毒检测鼠干扰素。结果发现，灰树花多糖能够促进ＮＤＶ诱生干扰素。各实验组、ＮＤＶ对照组和生理盐水对照组的干扰素活性分别为４４．５，４５．０，２９．０，１２．０和２．０Ｕ／ｍｌ，据此认为，灰树花多糖抗病毒感染的作用可能由干扰素系统介导，但也可能有其他机理参与。

NIH mice were orally fed by intubating Grifolan polysaccharide at the dose of 2000,1000,and 500 mg/kg of body weight daily for 7 days.They were injected with 0.5 ml of NDV(Newcastle disease virus)suspension containing 1280 HAU/ml(hemagglutination units).After 6hours the mice were bled and their sera assayed.for the production of interferon.The murine sera were assayed on L 929 cells and VSV.It wasfound that the Grifolan polysaccharide was able to enhance the production of interferon induced by the NDV.The interferon activity of the three test groups and NDV, saline injected controls were 44.5,45.0,29.0,12.0 and 2. 0 U/ml respectively.These results suggested that the protective effects of Grifolan polysaccharide against virus infection might be mediated by the interferon system. However,other mechanisms might also be involved.