大鼠全脑缺血及再灌注对不同脑区GSH含量及GSH－Px活性影响

The effects of ischemia andreperfusiong on glutathione content and glutathione peroxidase activity in different region o ratbrain.

采用闭塞大鼠四条动脉的全脑缺血模型，观察缺血３０ｍｉｎ及再灌注不同时期某些脑区中ＧＳＨ含量及ＧＳＨ－Ｐｘ活性的变化。结果显示，与对照组相比，缺血３０ｍｉｎ后ＧＳＨ、ＧＳＨ－Ｐｘ均有下降，随再灌期延长，ＧＳＨ含量逐渐回升，至再灌注４８ｈ恢复至原有水平；而ＧＳＨ－Ｐｘ活性在再灌注初期（２ｈ）有所回升，但至再灌注２４ｈ再度出现下降。这种变化趋势，在大脑皮层和海马中表现显著，而在丘脑和下丘脑中差异不显著或幅度较小。表明大脑皮层和海马是缺血神经元选择性易损区。Ｐ＜０．０１（与对照组相比）ｎ＝６由表１可知，与对照组相比，缺血３０ｍｉｎ时，ＧＳＨ、ＧＳＨ－Ｐｘ显著降低（Ｐ＜０．０１）。缺血３０ｍｉｎ后再灌注期间，随时间延长，ＧＳＨ逐步回升，至４８ｈ已基本恢复到对照组水平。ＧＳＨ－Ｐｘ在再灌注初期（２ｈ）略有回升，至２４ｈ又进一步降低，甚至低于缺血３０ｍｉｎ时；至４８ｈ有所增高，但显著低于对照组（Ｐ＜０．０１）。２．２缺血和再灌注时丘脑和下丘脑ＧＳＨ、ＧＳＨ－Ｐｘ变化由表２可知，与对照组相比，缺血３０ｍｉｎ时，ＧＳＨ含量有所下降，并随再灌注时间延长而升高，至４８ｈ己恢复原有水平，各组间均无显著差异。缺血３０ｍｉｎ表２缺血和?

By a rat brain ischemia model,with four vessels occlusion,we observed the changes of glutathine content and glutathione peroxidase activity during 30min ischemia and at different reperfusion stages.The results showed that the GSH content and GSH-Pxactivity were declined as compared withthe control.When reperfusion was prolonged after 30 min of brain ischemia,the GSH content was raised again gradually and restored to the original level after reperfusion for 48 hours; whereas the GSH-Px activity was enhanced after reperfusion for 2 hours and refallen after forreperfusion 24 hours.These changes wers significant in the cerebral cortex and hippooampus,but in thalamus and by pothalamus were not significant orwithin a narrower range.It suggested that the hippocampus and cerebral cortox were the seleotive vulnerability of neuron ischemia in the region of the brain.