大鼠背根切断和电针后脊髓胶状质内鸟核苷酸调节蛋白的改变(免疫组织化学观察)

Change of the Guaning Nucleotide-regulating Protein in Substantia Gelatinosa of Spinal Gord of Rats After Unilaterat Transection of Dorsal Roots and Electroacupuncture: An Immunohistochemical Observation

应用兔抗岛核苷酸调节蛋白的功能单位（即：Go蛋白—αo）多克隆抗血清，采用免疫组织化学方法观察大鼠脊髓内αo免疫反应性（αo－IR）对Go蛋白进行定位，及其在切断脊神经背根后和电针镇痛时的改变。结果发现正常鼠和对照组鼠脊髓后角浅层内均出现强的αo－IR，第Ⅱ层（胶状质）最为密集；外侧脊核区也出现αo-IR纤维网。切断背根后和电针镇痛时，胶状质内αo-IR及其光密度定量均明显降低。表明胶状质内与一级伤害性刺激传入有关的神经元末梢内Go蛋白部分来源于一级感觉神经元，及类“抑制”型Go蛋白的释放增加（αo－IR减弱），提示Go可能参与调控一级感觉的传递及电针镇痛。

The Subunit αo of guanine nucleotide-binding protein(rabbit polyclonal antiserum 9120, K,J.Chang.USA),in the areas of rat spinal cord was localized by immunohistochemical PAP methods,and the changes of αo-immunoreactivity (αo-IR ) in the substantia gelatinosa (SG) were observed after unilateral tranartion of dorsal roots and electroacupuncture.Intense αo-IR was presented in Rexed lamina Ⅰ to Ⅲ of the dorsal horn,and the highest αo-IR in lamina(SG), which was found than that in the normal and control animals.Iateral spinal nucleus of rat revealed higher density of αo-IR containing fiber networks.Following unilateral transection of dorsal roots and pain threshold increased significantly after electroacupuncture applied at the point "Huantiao",αo-IR was markedly decreased than that in the normal and control group in those areas.The optic density(OD) of αo-IR were measured,which decreased more markedly in opration and electroacupuncture group than that normal and control gruoup.The results mentioned above indicate that Go-protein in terminals of primary nociceptive neurons of SG decreases markedly after dorsal roots transected and electroacupuncture.It was inferenced that partial Go-protein probably originates from the primary sensory neurons, and the release of "inhibitory"of Go may increase(with weaker αo-IR).In a work. Go might playimportant roles on the primary afferents informational regulation and electroacupuncture analgesia of the regulation