辣椒素敏感神经元及针刺穴位对后角乙酰胆碱释放的影响(ACh活度在体测定)

Influences of Capsaicin and Electroacupuncture at "Point" on Acetylcholine(ACh) Release in the Spinal Dorsal Horn of Rats

本文采用脊髓后角乙酰胆碱（ACh）离子选择电极在体连续、原位、实时、动态的观察方法研究针刺穴位后；脊髓后角表面滴注Cap；切断一侧背根；一侧脊髓后角予以SP抗血清后对背角ACh活度的影响。结果发现针刺穴位可提高后角深浅层内ACh活度；Cap可促使后角内ACh的释放；切一侧背根使之溃变，则Cap促ACh释放的作用取消；予以SP抗血清，Cap促ACh释放的作用部分抑制，提示：（1）一级感觉传入中的Cap敏感神经中有ACh存在；（2）含SP的一级感觉传入神经对背角ACh的释放具有调节作用。

The dynarnic changes of ACh activity in the spinal dorsal horn of rats were recorded by ACh ion selective microelectrodes. The influences of applying capsaicin to the spinal surface, adm1nistering anti - SP surum to the interior of dorsal horn, cutting of f dorsal roots and electroacupuncturing at 'points' on the ACh activity were studied. The results showed that electroacupuncture at 'points' and capsaicin could facilitate ACh release of the spinaI dorsal horn, it was fully inhibited by cutting off dorsal roots or obviously inhibited by administering anti-SP surum, The above results indicated that ACh rnight participate in the prirnary afferent of painsensation and acupuncture sensation as a neurotransmitter and SP might adjust the relese of ACh in spinal dorsal horn