外周应用密胆碱对针刺调节免疫反应的影响

Effect of HC-3 on Electroacupuncture-induced Immunoregulation

前文研究提示外周交感神经在电针调节免疫反应中起着重要作用[4]。本研究的目的在于探讨副交感神经在其中所起的作用。实验用绵羊红细胞予先四天免疫小鼠，电针前3小时分别给小鼠腹腔注射生理盐水或密胆碱，发现盐水对照组小鼠电针后脾淋巴细胞转化率、IL－2含量及胸腺细胞数均显著增加（P＜0．01、＜0．001、＜0．05），而其他免疫指标未见明显变化。应用密胆碱阻断外周ACh生物合成后，小鼠脾淋巴细胞转化率及IL－2含量显著降低（P＜0．05、＜0．001），其他指标也无明显变化。若用密胆碱后再给予电针刺激，则小鼠脾淋巴细胞转化率及IL－2含量与单独HC－3组比较也未见明显变化。但值得注意的是药物电针组小鼠的其他多种免疫指标如脾重量、脾指数、脾细胞数、脾溶菌酶、胸腺重量、胸腺指数、胸腺溶菌酶等均呈下降或减少，并显著低于盐水对照组水平。以上结果说明：针刺兴奋副交感神经通过释放ACh递质对免疫反应有一定的促进作用。

Our previous study suggested that peripheral sympathetic nerve played an important role in the electroacupuncture (EA)-induced irnrnunomodulation. The aim of this study is to explore the role of peripheral parasympathetic nerve in it. All mice are preimmunized with SRBC (sheep red blood cell) 4 days before experiment. The animals in different groups were injected i. p. with Hemicholine-3 (HC-3, an ACh synthesis blocker) or physiological saline 3 hr before EA. The main results are as follows: 1. In saline group, the LTT (lymphocyte transformation test), IL-2 (interleukin-2 ) of spleen and lymphocyte number of thymus are rnarkedly increased, and the other immune parameters have no change after EA.2. In HC-3 control group, the LTT, IL-2 are decreased significantly and the other immune parameters have not shown influence.3. In the HC-3 with EA group, the magnitude of LTT, lL-2 did not significantly change as compared with HC-3 alone, it is especially noteworthy that the other unchanged parameters by HC-3 such as spleen weight, spleen index, thymus weight, thymus index, number of spleen cells, spleen LZM(lysozyrne) and thymus LZM are clearly decreased after EA.The above results suggest that peripheral parasympathetic nerve may play some prornoting effect in the EA-induced immunomodulation. Its effect may be mediated by ACh released from parasympathetic nerve endings