土人参原生质体培养再生植株

PLANT REGENERATION FROM PROTOPLASTS OF TALINUM PANICULATUM

分别由土人参（Ｔａｌｉｎｕｍ ｐａｎｉｃｕｌａｔｕｍ （Ｊａｅｑ．） Ｇａｅｒｔｎ．）组织培养再生苗的叶片和幼茎诱导的愈伤组织游离出原生质体．叶肉原生质体在培养中未能进行正常分裂，存活不过１ 周．愈伤组织原生质体在Ｐ４ 培养基中（Ｋ８ｐ＋ ２，４－Ｄ ０．２ ｍ ｇ／Ｌ＋ ＮＡＡ １．０ ｍ ｇ／Ｌ＋ ＺＴ ０．５ ｍ ｇ／Ｌ＋椰乳５０ ｍ Ｌ／Ｌ＋ 葡萄糖０．５ ｍ ｏｌ／Ｌ）培养３ ｄ 开始第一次分裂，培养７ ｄ 时分裂频率为３６．７％ ． 愈伤组织再生率在液体培养中为０．３１％ ，在双层培养中为０．３４％ ． 愈伤组织在含有较低浓度的６－ＢＡ 的分化培养基上分化出不定芽． 幼苗生根后移栽到花盆中继续生长，２～３个月后开花结实，长出粗壮的肉质根． 再生小植株在试管中继代培养２～３ 个月开花结实． 研究结果还表明∶（１）愈伤组织在液体培养基或增殖培养基中培养时间过长，或继代次数过多均不利于分化．（２）较低浓度的６－ＢＡ （０．５～０．７ ｍ ｇ／Ｌ）对愈伤组织的分化是合适的．（３）ＧＡ３ 对幼苗的发育有促进作用．

The protoplasts of Talinum paniculatum (Jaeq.) Gaertn. were isolated from leaves and calli. The mesophyll protoplasts did not undergo normal division and lived one week at the longest in culture. However, the callus protoplasts, cultured in P 4 medium (K8p+2,4 D 0 2 mg/L, NAA 1 0 mg/L, ZT 0 5 mg/L, coconut milk 50 mL/L, glucose 0 5 mol/L), underwent first division after 3 d of culture. The division frequency was 36 7% after 7 d of culture. The regeneration frequencies of callus were 0 31% in liquid culture and 0 34% in double layer culture. Shoots differentiated on regeneration media and rooted on R 3 and R 7 media. Mature plants were obtained 2～3 months after transplanting the protoplast derived plantlets into flower pot or successive subculturing in test tubes. The results also indicated that: (1) Too long a period of callus culture in liquid medium or in solid proliferation medium was unfavorable to differentiation. (2) Low concentration of 6 BA in medium was suitable for callus differentiation. (3) GA 3 promoted development of young adventitious bud. (4) Multi effect triazole significantly strengthened sprout and root development in test tube cultures.