摘要
由多种盐组成的复合渗透稳定剂用于分离蓝藻原生质球的效力与单一盐溶液相比较，其作用多数显示加强，但对原生质球稳定性的影响随盐类组合而异。若干种盐类，如酒石酸铵、硫酸铵和硫酸镁，对蓝藻细胞壁表现一定的降解作用，以酒石酸铵作用最强，可用于分离原生质球。此种原生质球透明度较差。
The compound osmotic stabilizers consisted of several salt solutions exerted a greater effect on the isolation of blue green algae spheroplasts than a single salt solution. However, the effect of compound osmotic stabilizers on the spheroplast stability could be multiphasic. Some osmotic stabilizers, such as the solution of (NH 4) 2C 4H 4O 6, (NH 4) 2SO 4 and MgSO 4, exerted degradation on cell walls of the blue green alga; among which the (NH 4) 2C 4H 4O 6 solution (0 15 mol/L) had the greatest degradation resulting in formation of spheroplasts. The spheroplasts were sensitive to hypotonic condition but were less transparent.
基金
湖北省自然科学基金
