北京地区一种侵染菊花的线条病毒

IDENTIFICATION OF A FILAMENTOUS VIRUS INFECTING CHRYSANTHEMUM

从北京地区菊花病毒病株上分离到一种线条状病毒CA分离物。经寄主范围、传播方式、汁液体外抗性、外壳蛋白分子量、粒体大小和在细胞中产生的内含体研究结果分析,此病毒为Potyvirus成员。沉淀反应、免疫双扩散反应和免疫电镜技术检测证明CA分离物与PVY无血清学相关性。CA分离物已制备抗血清和提纯IgG,并应用于品种菊组培苗脱毒检测工作。

A filamentous virus isolate, CA, was isolated and identified from chrysanthemum showing mottle symptom in Beijing area. Chenopodium quinoa and Nicotiana tobacum 'xanthi' were the best indicator and propagation species. Isolate CA could be transmitted by Myzus persicae in non-persistant manner efficiently. The properties of crude sap were: TIP = 50 -60℃, DEP=10-3-10-4,LIV=2~3 days. The purified CA preparetion contained flexuous particles 692nm in average and one type of coat protein of Mr 32. 3×103. Pinweel and bundle inclusions were found in the infected leaf cells. CA antiserum with microprecipitin titer of 1:512 was obtained. The IgG was used for the ELISA test of tissue culture of chrysanthemum. According to the result of identification isolate CA is considered as a member of Po-tyvirus. The tests of double diffusion, Precipitin, Latex and ISEM demonstrated no sero-logical relationship between CA and PVY.