蚕豆叶片SOD活性监测大气SO_2污染的可行性研究

STUDIES ON THE FEASIBILTY OF SOD ACTIVITY AS BIOINDICATORS OF SO_2 POLLUTION

本文从实验室熏气和野外大气暴露两方面对利用蚕豆叶片ＳＯＤ活性评价和监测大气ＳＯ＿２污染的可行性进行了研究。低浓度ＳＯ＿２（０．１３１２、０．２６０１ｍｇ·ｍ￣（－２））处理，引起叶片ＳＯＤ活性升高，一定时间后，ＳＯＤ活性趋于稳定，且０．２６０ｍｇ·ｍ￣（－２）ＳＯ＿２处理时，ＳＯＤ活性较高，表现出ＳＯＤ活性增量与ＳＯ＿２浓度相关，为利用ＳＯＤ活性监测和评价ＳＯ＿２污染提供了可能性，大气暴露试验结果表明ＳＯＤ活性与大气硫酸盐化速率存在极显著的相关性。利用ＳＯＤ活性和大气硫酸盐化速率分别对大气ＳＯ＿２污染程度进行了评价，结果基本一致，并根据ＳＯＤ活性估测了大气硫酸盐化速率，符合程度较高，置信分析表明估测结果可信，以上结果表明，利用蚕豆叶片ＳＯＤ活性监测和评价大气ＳＯ＿２污染是可行的。

Fumigation and ambient expiosing experiments were conducted to evaluate the feasibility of using SOD activity in the leaves of broadbean (Vicia faba ) as bioindicators of SO_2 pollution. Pumigation experiments were conducted indynamic fumigation systems with SO_2 concentrations of 0.1312 and 0.2601mg·m￣(-3) ,respectively. The SO_2 fumigated leaves had higher SOD activities thanthe control,and higher concentration(0.2601 mg·m￣(-3)) of SO_2 caused gteaterincrease in SOD activity than lower concentration (0.1312mg· m￣(-3)).The SODactivity in SO_2 fumigated leaves increased gradually at the beginning andreached a relatively stable stage after several days of treatment. Ambient exposing experiments were conducted with the form of plant-meter in 8 sites for 21 days. The SOD activity correlated significantly withthe rate of sulfatization (RS) in the air at the level of P<0.01. The degreesof SO_2 pollution at sample sites were evaluated with SOD activity and RSrespectively.RSs were also estimated by SOD activity,and then comparedwith the actually detected values.The 95% confidence interval was alsomade. All of our results ruggest that it is feasible to use SOD activity inthe broadbean leaves as bioindicators for SO_2 pollution.