摘要
利用水稻 Oryza sativa L.,2 n=2 4 1 2对染色体上的 5 4个 DNA限制性片段长度多态性 RFL P 标记 ,研究了它们在籼粳杂种 窄叶青 8号 / 京系 1 7 F2 群体中的分离 .结果表明 ,2 5 .9%的 RFLP标记的分离显著或极显著地偏离了预期的孟德尔比例 1∶ 2∶ 1 而表现为异常分离 .F2 群体中 3类 RFL P基因型中 窄叶青 8号 类型明显偏多 ,其基因频率为5 2 .1 % .同时还发现了 3个异常分离的染色体热点 ,即第 3染色体上的 RG2 2 7- RG369,第 7染色体上的 RG678- RG5 1 1 - RG5 2 8,以及第 1 2染色体上的 RG4 63- RG32 3,它们可能与导致异常分离的配子体基因座位有关 .
The segregation ratio of RFLP markers in an F 2 population from indica “Zhaiyeqing 8” and japonica “Jingxi 17” of rice (Oryza sativa L., 2n=24) was studied using 54 RFLP markers distributed on 12 chromosomes. Distorted segregation was found in 25.9% of the marker tested, which was indicated by significant deviation from the expected Mendelian segregation ratio ( 1∶2∶1) at 5% or 1% level. Among the three RFLP genotypes of the F 2 population “Zhaiyeqing” 8 genotype was significantly more than the expected, and its gene frequency was up to 52.1%.Three positions for distorted segregation were found on chromosome 3 (RG227 RG369), 7 (RG678 RG511 RG528) and 12 (RG463 RG323). These positions could be related to gametophyte loci responsible for the distortion.
关键词
异常分离
染色体分布
籼粳杂种
水稻
RFLP
标记
Restriction fragment length polymorphism markers
Distorted segregation
Chromosome distribution
Indica/japonica hybrid
Oryza sativa
