摘要
Cu2 + 抑制钝顶螺旋藻 ( Spirulina platensis)完整细胞中电子传递活性 ( H2 O→ MV) ,并抑制 PS 的放氧活性。低浓度的 Cu2 +处理 ,使钝顶螺旋藻细胞中藻蓝蛋白荧光发射峰位置蓝移、发射强度改变 ,表明藻胆体中能量传递发生了变化。Cu2 +处理纯化的藻蓝蛋白 ,使其在长波区 ( 61 6— 62 0 nm)吸收减小、荧光发射峰蓝移 ( 64 7nm→ 64 3nm) ;而变藻蓝蛋白不受影响 ,说明 Cu2 + 选择性作用于藻蓝蛋白。
Addition of Cu 2+ at low concentrations, to intact cells of the cyanobacterium, Spirulina platensis, at room temperature, caused an enhancement in intensity of fluorescence emitted by phycocyanin and induced a blue shift at the emission peak, both of which indicated changes in energy transfer within the phycobillisomes.Cu 2+ also suppressed the whole chain electron transport activity (H 2O→MV) and water splitting activity of the photosystem Ⅱ.When isolated phycocyanin and allophycocyanin were exposed to very low concentrations of Cu 2+ ions, C phycocyanin but not allophycocyanin, exhibited decrease not only in the absorbance in the longer wavelength (616—620 nm) region, but also in the fluorescence emission intensity at 647 nm accompanied by a blue shift to 643 nm.These results suggested that Cu 2+ selectively bleach C phycocyanin.
基金
四川大学校基金
