马尾松成熟合子胚的体细胞胚胎发生和植株再生

SOMATIC EMBRYOGENESIS AND PLANTLET REGENERA TION FROM CALLUS OF MATURE ZYGOTIC EMBRYOS OF MASSON PINE

采用马尾松（Ｐｉｎｕｓｍ ａｓｓｏｎｉａｎａ Ｌａｍ ｂ．）成熟合子胚为起始外植体，在含２，４－Ｄ １０ ｍ ｇ／Ｌ，ＫＴ和ＢＡ 各４ ｍ ｇ／Ｌ的ＤＣＲ培养基上得到胚发生培养物。将白色半透明的愈伤组织（含早期原胚）在含２，４－Ｄ１．０ ｍ ｇ／Ｌ，ＫＴ和ＢＡ 各０．４ ｍ ｇ／Ｌ的ＤＣＲ培养基上保持并增殖。在附加９０００ ｍ ｇ／Ｌ肌醇的ＤＣＲ高渗培养基上得到粗壮的后期原胚。ＡＢＡ 和活性炭同时使用能促进子叶胚的形成，最高频率为３５．１％ 。在无激素培养基上。

Embryogenic cultures were initiated from mature zygotic embryos of masson pine (Pinus massoniana Lamb.) on DCR medium supplemented with 2,4 D 10 mg/L, KT and BA each at 4 mg/L. Pale and translucent calli with early stage proembryos were maintained and multiplicated on DCR medium supplemented with 2,4 D 1 0 mg/L KT and BA each at 0 4 mg/L.Robust late stage proembryos were obtained when the calli were cultured on DCR medium containing 9000 mg/L myo inositol.Abscisic acid and activated charcoal promoted the formation of cotyledonary embryos at the highest frequency of 35 1%.Mature somatic embryos could germinate and develop further into plantlets when they were isolated and cultured on a hormone free DCR medium.