钛对离体黄瓜子叶几种生理指标的影响

EFFECT OF TI-CHELATOR ON THE EXPANSION, SYNTHESIS OF CHLOROPHYLLY AND CAROTENOID OF EXCISED CUCUMBER COTYLEDONS

将黄瓜种子在(25±1)℃暗中或光下萌发,其子叶离体后,用不同浓度的螯合钛处理,结果表明:螯合钛对黄瓜子叶的扩张、叶绿素合成、类胡萝卜素的积累、核酸含量的增加有明显的促进作用,其中在光照条件下经螯合钛处理后的离体黄瓜子叶,其鲜重和核酸的增加比暗中明显,且光下比暗中螯合钛对DNA的合成影响更为显著。

Seed of cucumber (cucumis satiwus L, Var. Jin Yan No.4) were germinated on filter paper saturated with distilled water at (25+1)C in dark or in light for five days. The excised cotyledons were in Ti-chelator solution. The fresh weight, contents of the carotenoid, chlorophyll, RNA and DNA of cotyledons were determined. The results showed that there were significant promotion in exhancing the expansion of cotyledons, the accumulation of carotenoid, the synthesis of chlorophyll, and increase of nucleic acid. The fresh weight and content of nucleic acid of excised cucumber cotyledons treated by Ti-chelator in light was greater than in dark. The effect of 2ug/mL Ti-chelator on DNA, RNA contents was a little bigger than 4ug/mL Ti-chelator, and the effect of Ti-chelator on DNA synthesis in light was great than in dark.