五虎汤对呼吸道合胞病毒复制的抑制作用研究

Inhibition of Respiratory Syncytial Virus by Wuhutang, A Traditional Chinese Prescription Composed of Five Medicinal Herbs

传统治喘名方五虎汤在体外和活体内均具有抑制呼吸道合胞病毒作用。在Hep-2细胞单层上，五虎汤抑制50％以上病毒空斑的浓度为1：80，在此一浓度，病毒繁殖量下降10-2．4TCID50，病毒核酸合成下降率约55％。方中麻黄具有抗RSV活性，其抑制50％以上病毒空斑浓度为1．6mg／ml。在动物体内，本方通过雪化吸入或口服，能降低小鼠肺组织内RSV滴度，促使小鼠肺组织RSV提前消失，并能促进小鼠肺组织炎症吸收。

Wuhutang, a well known traditional Chinese. herbal decoction for the treatment of as thma was found to havc an antiviral effect on respiratory syncytial virus (RSV ) both invitro and in vivo. A 50% reduction of plaque. formation was observed op Hep-2 cell monolayer at a concentration of 1:80. At such concentration, the. value of lg TC ID50 of. Viralyield was 2, 1, and 55% RNA synthesis of RSV was inhibited. It was found that among thefive medicinal herbs in prescription Ephedra sinica Stapf is responsible for the antiviralactivity on RSV. IC50 for plaque inhibition was found to be 1.6mg/ml. In animal model,Wuhutang singn ficantly reduced the titre of RSV and accelerated RSV clearance in mice,and alleviated viral pneumonitis by either oral or aerosol route.