缺血及再灌流时不同脑区磷脂酶A_2活性的变化及作用探讨

Study on PLA_z activities in different regions during brain ischemia reperfusion

本文利用大鼠４血管阻断法（４ＶＯ）建立脑缺血再灌流模型，分别检测假手术组，缺血１５ｍｉｎ组，缺血１５ｍｉｎ后再灌流１ｈ、６ｈ、２４ｈ、７２ｈ和１６８ｈ各组不同脑区的磷脂酶Ａ_２（ＰＬＡ_２）活性变化。发现海马和皮质下脑区ＰＬＡ_２活性在再灌流早期显著升高，但随再灌流时间延长其活性反而低于假手术组；而皮质区ＰＬＡ_２活性在再灌流早期虽然也升高，但很快接近假手术组。表明ＰＬＡ_２活性改变有着区域性差别，参与并影响了缺血再灌流后选择性神经元坏死的病理过程。

The brain model of ischemia and reperfusion was set up by rat 4VO method, then the PLA_z activities in different brain regions of control group, ischemia 15min group, reper fusion 1h,6h,1d,3d and 7d groups were tested. The results revealed that PLA_z activities of hippocampus and subcortical region went up at early reperfusion, but reduced to the less level than control group as reperfusion continued; and that PLA_z activity of cortical region went up too at early reperfusion, but reduced to the control level soon. It suggested that the rules of PLA_z activity changes are different in several regions. and that PLA_z plays an important role in selective neuron necrosis.