油桐尺蠖核型多角体病毒感染Bs484细胞的研究

Studies on Infection of Buzura suppressaria Nuclear Polyhedrosis Virus in Bs484 Cells

本文报道利用油桐尺蠖核型多角体病毒感染处于不同培养基及不同培养时间的Ｂｓ４８４细胞的部分感染效果和特征。结果表明，细胞培养基的类型和细胞传代后的时间对病毒感染率和多角体产量以及其它感染特征都有比较明显的影响；经过比较，处于Ｇｒａｃｅ培养基组的传代后三天的Ｂｓ４８４细胞是一个理想的ＢｓＮＰＶ感染受试系统。同时发现，健康的油桐尺蠖血淋巴具有提高细胞活性及病毒感染效果的作用。

he part of effects and characters of infection with Buzura suppressaria nuclear polyhedrosis virus(BsNPV)for Bs484 cells in the different media and cultural time were reported in this peper.The re sults showed that the types of media and the time after passage for Bs484 cells could produce distinct in-fluences on the rate of viral infection and the ytelds of polyhedra from BsNPV-infected Bs484 cells as ell as the other infective characters,The Bs484 cells of three days after psaseage simultaneously in the group of Grace media was a relatively pragmatic testing system of infection with BsNPV through expor imental comparision. In addition,this exporiment also found that the healthy hemolymph from the o-riginal insects could increase the cellular activity and the effects of viral infection.