兔出血症病毒主要结构多肽的氨基酸分析

Amino Acid Analysis of the Major Structural Polypeptides of Rabbit Haemorrhagic Disease Virus

兔出血症病毒主要结构多肽的氨基酸分析王恒安，杜念兴，徐为燕（南京农业大学，南京２１００９５）关键词兔出血症病毒，结构多肽，氨基酸分析有关兔出血症病毒（ＲＨＤＶ）结构多肽的报道很多，有认为只有１条，有报道４条的，也有报道多达６条的。但其主要结构多肽为分...

abbit haemorrhagic disease virus(RHDV)was purified from infected liver by freezing and thawing,chloroform extraction,PEG 6 000 precipitation and Sepharose-4B chromatography.Six polypeptides ,with the molecular weights of 64.5kD,61.4kD, 57.3kD,54.0kD.29.2kD and 28.1kD weredetermined by immuno blotting to be viral structural polypeptides.The major strucrural polypeptide(64.5kD),purified electrophoretically was analysed by hydrochloricacid lysis and fourteen kinds ofamino acids were found,of which,the base,neutral and acidic amino acids accounted for 75.14%,21.16% and 3.60%,respectively.