肝硬变中丙型肝炎病毒C_（33）c抗原及HBxAg的分布及意义

Detection of the HCV C_(33c) Antigen and HBxAg in Liver Cirrhositic Tissues by Immunohistochemical Techniques

应用抗ＨＣＶＣ＿（３３ｃ）抗原２Ｂ６株单克隆抗体和抗ＨＢｘＡｇ多克隆抗体。以ＡＢＣ法对８６例肝硬变组织进行ＨＣＶ及ＨＢＶ相关抗原定位研究，ＨＣＶＣ＿（３３ｃ）抗原及ＨＢｘＡｇ在肝硬变中的阳性率分别为７６．７％及６２．８％，Ｃ＿（３３ｃ）抗原和ＨＢｘＡｇ阳性占所检病例８８．４％，二者同时阳性为５１．２％，ＨＣＶＣ＿（３３ｃ）抗原位于肝硬变组织的肝细胞胞桨内，充满整个胞桨，细胞核及胞膜未见阳性；阳性细胞呈弥漫、局灶及散在分布三种形式，以弥漫、局灶分布为主。部分病例阳性细胞周围有较多的淋巴细胞、单核细胞浸润。并发现ＨＣＶＣ＿（３３ｃ）抗原位于小胆管上皮细胞内。结果提示ＨＣＶ感染致肝细胞损伤可能是免疫反应参与的免疫损害。除ＨＢＶ以外，ＨＣＶ感染在我国肝硬变发生中起一定作用，在肝硬变组织中ＨＣＶ、ＨＢＶ重叠感染较为普遍。

he HCV C_(33c) antigen and HBxAg were detected with monoclonal antibod ies against C_(33C) and Poly-clonal antibedies agalnst HBxAg by immunohistochemical techniquee in 86 paraffin-embeded sectionsof liver cirrhositic tissues,The rate of the HCV C_(33c) antigen and HBxAg positive was 76.7%(66/86)anct 62.8%(54/86)respectively.The rate of either the HCV C_(33c) or HBxAg positive was 88.4%(76/86).The rate of both the HCV C_(33C) and HBxAg positive was 51.2%(44/86).The HCV C_(33c) antigenwas clearly stained in the cytoplasm of hepatocytes but not in the nucleus or cell membrane.The posi-tive signals were distributed diffusely in the cytoplasm of hepatocytes,The HCV C_(33c) antigen positivecells were distributed in three ways:diffuse,cluster and patchy,but mainly diffuse and cluster.Therewere a lot of inflammatory cells accumulated around the HCV C_(33c) antigen positive cell.THeHCV C_(33c) antigen were also detected in the cytoplasm of small cholangic epithelial cells;Oufresults suggests that immune reactions may be involved in the HCV induced hepatocyte injury,To a certain extent,HCV infection may play a role in the development of liver cirrhosisbesides HBV infection,HCV and HBV double infection was very commonn in liver cirrhosis.