兔出血症病毒结构多肽分析

The Analysis of the Structural Polyeptides of Rabbit Hemorrhagic Disease Virus

粗提病毒经Ｓｅｐｈａｒｏｓｅ４Ｂ层析后，获得纯化的兔出血症病毒（ＲＨＤＶ）。提纯病毒经过ＳＤＳ－ＰＡＧＥ经考马斯亮蓝染色显示Ａ、Ｂ、Ｃ、Ｄ、Ｅ、Ｆ和Ｇ７条多肽，凝胶扫描显示Ａ为ＲＨＤＶ主要结构多肽。用多抗和单抗作免疫转印分析，证实Ａ、Ｂ、Ｃ、Ｄ、Ｅ、和Ｇ为结构多肽，此６条结构多肽间的抗原关系十分密切。

he RHDV virion comprised six sturctural polypeptides as analysed by sodium dodecylsulfate-polyacrylamide gel electrophoresis, including A(molecular weight 60.12 kD),B(58. 27kD),C(54.74kD),D(53. 01kD),E(51. 10kD) and G(25. 90kD).The major polypep-tide A(including polypeptide B constituted about 54.7% of the total viral proteins. polypep-tide C constituted 1 7.7%, polypeptide D and E were about 22.7%and polypeptide G wereabout 4.9%. By western blotting with rabbit antiserum,polypeptides A、B、C、D、E and Gwere detected. by using monoclonal antibodies AA8-1、CG4-1 and CH3-1,all the six polypep-tides could be recognized,indicating that these polypeptides shared extensive antigenic rela-tionship.