摘要
粗提病毒经Sepharose4B层析后,获得纯化的兔出血症病毒(RHDV)。提纯病毒经过SDS-PAGE经考马斯亮蓝染色显示A、B、C、D、E、F和G7条多肽,凝胶扫描显示A为RHDV主要结构多肽。用多抗和单抗作免疫转印分析,证实A、B、C、D、E、和G为结构多肽,此6条结构多肽间的抗原关系十分密切。
he RHDV virion comprised six sturctural polypeptides as analysed by sodium dodecylsulfate-polyacrylamide gel electrophoresis, including A(molecular weight 60.12 kD),B(58. 27kD),C(54.74kD),D(53. 01kD),E(51. 10kD) and G(25. 90kD).The major polypep-tide A(including polypeptide B constituted about 54.7% of the total viral proteins. polypep-tide C constituted 1 7.7%, polypeptide D and E were about 22.7%and polypeptide G wereabout 4.9%. By western blotting with rabbit antiserum,polypeptides A、B、C、D、E and Gwere detected. by using monoclonal antibodies AA8-1、CG4-1 and CH3-1,all the six polypep-tides could be recognized,indicating that these polypeptides shared extensive antigenic rela-tionship.
出处
《中国病毒学》
CSCD
1995年第3期258-260,共3页
Virologica Sinica
基金
农业部八五课题
关键词
兔出血症病毒
结构多肽
分析
Rabbit hemorrhagic disease virus,SDS-PAGE,Immunoblotting