流行性出血热患者皮肤组织内病毒抗原及免疫复合物检测与意义

The Significance of Viral Antigen and Immune Complexes in Skin Tissues of Patients with Epidemic Hemorrhagic Fever

应用常规病理、免疫病理及超微病理技术，对３３例流行性出血热（ＥＨＦ）患者皮肤活检标本的病理变化及病毒抗原、免疫复合物进行观察，同时与血清病毒抗原、抗体及循环免疫复合物检出情况进行比较。在２３例ＥＨＦ患者皮肤微血管内皮细胞中检出病毒抗原，部分组织中可同时检出免疫球蛋白及Ｃ３，少数组织仅能检出病毒抗原或免疫球蛋白。配对血清小也可检出ＥＨＦ病毒抗原、抗体及循环免疫复合物。组织及血清免疫复合物形成与血清补体Ｃ３水平下降有关，组织内肥大细胞脱颗粒与血清ＩｇＥ水平升高相关，提示多种变态反应参与了流行性出血热的发病机制。

he techniques of pathology.immunohistochemistry and ultrastructural pathology were empluyed to study the pathological changes,specific virus antigens and immune complexes in the biopsy skin tissues of 33 cases of patients with epidemic hemorrhagic fever.The specific antigens,antibodies and circulatilng immune complexes in sera of the patients were also de-tected. T he specific viral antigens were detected in 23 skin samples of the patients and im-munoglobulins,complement component C_3 were also detected in some of the samples. How-ever,for a few samples,only the virus antigens or immunoglubulins were detected.The viral antigens,antibodies and immune complexes appeared in the sera of the patients showed some relationship with the severity and the phase of illness,Furthermore,we found that there were correlations between the consumption of C_3 and the formation of immune complexes,also the degranulation of dermal mast cells and the elevation of immunoglobulin E level in sera of the patients.These suggest that some allergy reaction have been involved in the pathugenesis of epidemic hemorrhagic fever.