武汉地区1988—1992年呼吸道合胞病毒流行株的抗原性分析

Antigenic Variation of Respiratory Syncytial Virus Isolated in Wuhan from 1988 to 1992

利用呼吸道合胞病毒（ＲＳＶ）的单克隆抗体（ＭｃＡｂ），通过间接免疫荧光法和免疫转印法对本室分离到的武汉地区１９８８一１９９２年的５６份ＲＳＶ毒株进行了分型，可将其分为Ａ、Ｂ两型和Ｂ１、Ｂ２亚型。流行病学分析显示：本地区ＲＳＶ的主要流行株，四年中有三年为Ｂ型株，不同于欧美等地；Ａ、Ｂ两型的分布还具有局灶化分布的特点；两型在性别、发病年龄之间无显著性差异。

ndirect immunofluorescense and Western-blot methods with seven home-made McAbs against RSV were used to distinguish RSV strains isolated in Wuhan from 1 988 to 1 992.These strains were distinguished as type A and B,further as subgruup B_1 and B_2.The RSV epidemic patterns in Wuhan were different from those of Europe and America,but were simi-lar to those of Japan.The prevalence of the two types even varied in two districts of Wuhan in the same year.It suggests the that RSV outbreaks should be regional and temporal clust-ing. No major differences in age and sex were found between the infants and young children infected with either type.