人工合成多肽检测抗HIV－1和HIV－2抗体的研究

A Study on the Detection of Antibodies to HIV Type 1 and Type 2 by Using Synthetic Peptidcs

采用固相法合成ＨＩＶ－１和ＨＩＶ－２两个多肽，建立了用混合多肽为包被抗原检测ＨＩＶ－１和ＨＩＶ－２感染的间接酶联免疫吸附法。检测４６份抗ＨＩＶ－１和ＨＩＶ－２抗体阳性血清标本以及９４份对照血清标本，与ＵＢＩ试剂比较，其阳性符合率为９７．８％，阴性符合率为１００％，总符合率为９９．３％。实验结果表明，此法可用于ＨＩＶ－１和ＨＩＶ－２感染的检测。

wo peptides of human immunodeficiency virus(HIV)type 1 and type 2 were synthe-sized by solid-phase method. An indirect enzyme-linked immunosorbent assay(ELISA)em-ploying these peptides as an antigen adsorbent was developed to detect the infections of HIV-1 and HIV-2.A comparison of our reagent with UBI reagent in detection of 46 positive serumspecimens and 94 negative control serum specimens for HIV-1 and HIV-2 showed that theconcident rate for positive was 97.8%, for negative was 100%,and the general coincidentrate was 99.3%. The results indicated that this method can be used in detection for infectionsof HIV-1 and HIV-2.