摘要
建立了香蕉试管苗黄瓜花叶病毒DAC-ELISA检测技术。应用0.1mol/L磷酸盐缓冲液(pH7.2,内含1%NaDIECA)作为样品制备缓冲液,检测灵敏度可达到1/5120(稀释度)。两年来检测了1287个样品,香蕉试管苗生产繁殖材料的带毒率为2.8%。
he present study established the direct antigen coating form for indirect ELISA (DAC-FLISA)for the detection of cucumber mosaic virus(CMV)in banana tissue culture. With aproper phosphate buffer solution,i.e.0. 1mol/L phosphate buffer(pH7.2,including 1%sodium diethyldithiocarbaniate) for preparing the crude plant sap to be detected,it was possi-ble to increase the sensitivity of DAC一ELISA to a dilution of plant sap at a rate of 1/5120.Atotal of 1 287 samples of productive material for banana tissue culture were detected forCMV.2.8%of them were found to be infected with CMV.
出处
《中国病毒学》
CAS
CSCD
1995年第3期254-257,共4页
Virologica Sinica
关键词
香蕉试管苗
黄瓜花叶病毒
检疫检验
Microprogation of banana,Cucumber mosaic virus,DAC-ELISA