香蕉试管苗黄瓜花叶病毒检疫检验技术研究

Studies on the Detection of Cucumer Mosaic Virus in Microprogation of Banana

建立了香蕉试管苗黄瓜花叶病毒ＤＡＣ－ＥＬＩＳＡ检测技术。应用０．１ｍｏｌ／Ｌ磷酸盐缓冲液（ｐＨ７．２，内含１％ＮａＤＩＥＣＡ）作为样品制备缓冲液，检测灵敏度可达到１／５１２０（稀释度）。两年来检测了１２８７个样品，香蕉试管苗生产繁殖材料的带毒率为２．８％。

he present study established the direct antigen coating form for indirect ELISA (DAC-FLISA)for the detection of cucumber mosaic virus(CMV)in banana tissue culture. With aproper phosphate buffer solution,i.e.0. 1mol/L phosphate buffer(pH7.2,including 1%sodium diethyldithiocarbaniate) for preparing the crude plant sap to be detected,it was possi-ble to increase the sensitivity of DAC一ELISA to a dilution of plant sap at a rate of 1/5120.Atotal of 1 287 samples of productive material for banana tissue culture were detected forCMV.2.8%of them were found to be infected with CMV.