中国对虾一种球状病毒的分离提纯及其核酸蛋白特性的研究

Isolation,Purification of Penaeus chinesis Parvovirus and Analysis of its Nucleic Acid and Protein

从感染致病的中国对虾（Ｐｅｎａｅｕｓｃｈｉｎｅｓｉｓ）中分离到一种球状病毒，其育径约为２０±４ｎｍ。进行人工感染实验，对虾死亡率为６６％；用脱氧核糖核酸酶（ＤＮａｓｅ），核糖核酸酶（ＲＮａｓｅ）及二苯胺染色法对病毒核酸进行处理，证明该病毒核酸为脱氧核糖核酸，用Ｓｌ核酸酶（Ｓｌｎｕｃｌｅａｓｃ）对该核酸进一步消化处理，进一步证实该病毒含单链ＤＮＡ。ＳＤＳ－ＰＡＧＥ结果显示，该病毒含４条结构多肽，其分子量分别为８６ｋＤ，７９ｋＤ，７０ｋＤ及２５．５ｋＤ。根据上述特性分析，该病毒可能属于细小病毒科（ｐａｒ－ｖｏｖｉｒｉｄａｅ），故暂定名为中国对虾细小病毒（ＰｅｎａｅｕｓｃｈｉｎｅｓｉｓＰａｒｖｏｖｉｒｕｓ简称ＰｃＰＶ）。

mall spherical virus with diameter of ca,20 nm was isolated from infected shrimps.Thevirions were icosahedron and naked. The infection exDeriment showed that the mortality was upto 66%.The viral nucleic acid was ssDNA after digested with DNase,RNase and Sl nuclease,Analysis of viral polypeptides by SDS-PAGE revealed that the virion had four structural polypep-tides with MW 86 kD,79kD,70 kD and 25.5 kD.These resul ts were in comformity with Par-voviridae,thus it was proposed that the virus be named Penaeus chinesis Parvovirus.