丙型肝炎病毒核心抗原高效表达及产物抗原性的研究

High Expr ession of HCV Cor e Antigen and the Study on the Recombinant Pr otein Antigenicity

用含谷胱甘肽转硫酶（ＧＳＴ）基因的ｐＧｅｘ－２Ｔ为表达载体在大肠杆菌中高效表达了含ＨＣＶ核心区部分基因片段（约１２２个氢基酸）的融合蛋白，表达产物Ｃ２７经测定占菌体总蛋白的３０％，Ｃ２７纯化后，用我国ＨＣＶ诊断试剂第二代血清参考品为标准与Ｇ１１核心抗原进行比较，结果显示二者具有相同的总体检出率和近似的抗原活性。因此，推测表达的部分区段基因可能是核心区重要功能区域。另外融合蛋白对提高重组蛋白的抗原活性和分离纯化等均有一定作用。

art of HCV core gene fragment is expressed in E.coli.The recombinant protein C27 is fu-sion protein with GST.The specific protein iS about 30%of total bacterial protein,After purifi-cation,the purity of the recombinant protein is more than 95%.We compared C27 antigen withC11 core antigen by EL ISA using the second generation panel sera of HCV EIA d iagnostic kit.The result showed that the two antigens had the same rate of sensitivity and specificity.So,weconcluded that the expressed gene fragment may be an important region in HCV core gene,Thefusion protein may also play an important role in increasing the antigenicity of the recombinant protem.