抗人丙二醛修饰低密度脂蛋白单克隆抗体的制备和免疫学性质

Preparation and the Immunological Characterization of Monoclonal Antibodies Against Malondialdehyde-modified Low Density Lipoprotein

本文报道两株抗丙二醛修饰的低密度脂蛋白单克隆抗体（ＨＭＬ１、ＨＭＬ２），两者均有较高的抗体效价．抗体类型为ＩｇＧ２ａ。单抗相加实验表明，ＨＭＬ１和ＮＭＬ２识别同一抗原位点。竞争性ＢＡ－ＥＬＩＳＡ实验显示：ＨＭＬ１能够识别丙二醛修饰的低密度脂蛋白、丙二醛清蛋白、丙二醛聚赖氨酸。但与天然低密度脂蛋白无交叉反应。推测ＨＭＬ１抗原位卢、与低密度脂蛋白在修饰过程中雨二醇和载脂蛋白Ｂ上的赖氨酸残基形成的共价结合物相关。

In this study, two monoclonal antibodies (McAb) against malondialdehyde-modified low density lipoprotein (MDA-LDL) were obtained, designated as HML1 and HML2 respectively, both of them can react to MDA-LDL with a high titer and belong to the subclass of IgG2a. The McAb additivity test shown that two McAb were against the same determinants of MDA-LDL. Solid-phase competitive biotinavidin EIA indicated that HML1 recognized MDALDL, MDA-alburnin, MDA-polylysine but not native LDL.It is postulated that the covalent aducts of MDA with lysine residues are involved the formation of MDA-derived epitopes on apolipoprotein B.