苯妥英锌抗脂质过氧化及对小鼠缺血心肌的保护作用

Anti-lipid peroxidation and protective effects of phenytoin zinc on mice ischemic myocardium

本研究观察苯妥英锌（ＰＨＴＺ）对异丙肾上腺素（ＩＳＯ）引起小鼠急性心肌缺血的保护作用，并与苯妥英钠（ＰＨＴＳ）比较。结果发现，与对照组相比，缺血组血浆ＭＤＡ含量升高１８．２％（Ｐ＜０．０５）；红细胞ＳＯＤ活力降低２７．８％（Ｐ＜０．０１）；全血ＧＳＨ－Ｐｘ活力降低１７．６％（Ｐ（０．０１），心肌组织出现明显缺血性损伤（Ｐ＜０．０１）。与缺血组相比，ＰＨＴＳ及ＰＨＴＺ组ＭＤＡ含量分别降低２６．３％（Ｐ＜０．０１）及２０．２％（Ｐ＜０．０１）；全血ＧＳＨ－Ｐｘ活力升高３３．８％（Ｐ＜０．０１）及２９．２％（Ｐ＜０．０１）；ＰＨＴＺ组ＳＯＤ活力升高３０．２％（Ｐ＜０．０１）。ＰＨＴＳ组ＳＯＤ活力低于对照组（Ｐ＜０．０１）和ＰＨＴＺ组（Ｐ＜０．０５），而与缺血组无差异，ＰＨＴＳ组ＧＳＨ－Ｐｘ活力高于对照组（Ｐ＜０．０５），ＰＨＴＳ组及ＰＨＴＺ组心肌组织缺血损伤明显减轻（Ｐ＜０．０１）。结果表明ＰＨＴＳ与ＰＨＴＺ均具有抗自由基引发的脂质过氧化反应、保护ＧＳＨ－Ｐｘ活力及保护缺血心肌作用，ＰＨＴＺ保护ＳＯＤ活力的作用可能与锌有关。

The protective effects of phenytoin zinc(PHTZ )and pbenytoin sodium(PHTS)on ischemic myocardium were investigated on experimental myocardial infarction model induced by subcutaneous injection of isoproterenol ( ISO)into mice.The results showed that(1)The MDA cont ents were increased by 18.2%(P<Q.05),SOD and GSH-Pxactivities decreased by 27.8%(P<0.01)and 17.6%(P<0. 01) respectively and severe my-ocardial ischemic lesion occurred In ischemic group,as compared with the control group;(2)MDA contents were decreased by 20.2%(P<0. 01 ) and 26.3%(P<0.01) respecti-vely,and GSH-Px activities were increased by 29.2%(P<0.01) and 33.8%(P<0.01 )respectively in PHTZ and PHTS groups, as compared with the ischemic group;(3 )Adm-inistration of PHTZ and PHTS protected myocardiam from ischemic lesion significantly;(4)SOD activities in PHTZ group were increased by 30.2%(P<0.01 ), as compared with ischemic grou.There was significant reduction of SOD in PHTS group,as com-pared with control group(P<0.01)and PHTZ group(P<0.05).The results indicated that PHTZ and PHTS possessed protective effects on blood GSH-Px activities, antilipid pero-xidation reaction and protective effects on ischemic myocardium,and that protective ef-fects of PHTZ on erythrocyte SOD activities was probably resulted from its zinc.