电离辐射对大鼠下丘脑和垂体内亮脑啡肽含量的影响

Effect of ionizing radiation on leucine-enkephalinof rat hypothalamus and pituitary

本文应用放射免疫分析（ｒａｄｉｏｉｍｍｕｎｏａｓｓａｙ，ＲＩＡ）方法检测４，８和２５Ｇｙ＿６０ＣｏＹ射线照射后大鼠下丘脑和垂体内亮脑啡肽（ｌｅｕｃｉｎｅ－ｅｕｋｅｐｈａｌｉｎ，Ｌ－ＥＮＫ）含量的变化。发现８Ｇｙ照射后２４和７２小时下丘脑Ｌ－ＥＮＫ含量分别为１３８．２３±１８．３２ｐｇ／ｍｇ和２１０．６８±２８．９９ｐｇ／ｍｇ，前者明显低于正常对照组（１７７．９２±２４．６８ｐｇ／ｍｇ）（Ｐ＜０．０１），后者明显高于正常对照组（Ｐ＜０．０５）。２５Ｇｙ照射后２４和４８小时下丘脑Ｌ－ＥＮＫ含量分别为１３１．６５±１０．６４ｐｇ±ｍｇ和１５２．６６±１４．０７ｐｇ／ｍｇ，均明显低于正常对照组（前者Ｐ＜０．０１，后者Ｐ＜０．０５）。２５Ｇｙ照射后４８小时垂体Ｌ－ＥＮＫ含量为９６．３７±１５．７４ｐｇ／ｍｇ，明显低于正常对照组（１２１．４１±１．９４ｐｇ／ｍｇ）（Ｐ＜０．０１）。

The changes of leucine-enkephalin( L-ENK)content of rat hypothalamusand pituitary were measured by radioimmunoassay after exposure to 4,8 and 25 Gy￣(60)Co γ-ray.The L-ENK content of hypothalamus 24 and 72 hours after 8 Gy irradiationwere 138.23 ± 18.32pg/mg and 210.68±28.99pg/mg, respectively. The former was signi-ficantly lower than that in the normal control group ( 177.92 ±24. 68pg/mg)(P<0.01).The latter was significantly higher than that in the normal control(P<0. 05 ).The L-ENK content of hypothalamus 24 and 48 hours after 25 Gy irradiation were 131.65 ±10.64pg/mg and 152.66± 14.07pg/mg respectively,which were significantly lower thanthat in the normal control.(former P<0. 01,latter P<0.05 ).48 hours after 25 Gyirradiation,the L-ENK content in pituitary was 96.37 ± 15.74pg/mg,which was signi-ficantly lower than that in the normal control group(121.41 ± 1.94pg/mg)(P<0.01 ).