大鼠免疫细胞的促肾上腺皮质激素受体的研究

ACTH receptor of rat immune cells

大鼠免疫细胞都有高、低亲和力两类ＡＣＴＨ受体。脾脏细胞高亲和力促肾上腺皮质激素（ＡＣＴＨ）受体的解离常数（ＫＤ）为０．１１ｎｍｏｌ／Ｌ，最大结合量（Ｂｍａｘ）为１．２ｆｍｏｌ／２×１０￣６细胞，３６６位点／细胞，低亲和力受体ＫＤ为５．７ｎｍｏｌ／Ｌ，Ｂｍａｘ为１４７ｆｍｏｌ／２×１０￣６细胞，４２０００位点／细胞。外周血淋巴细胞（ＰＢＬ）高亲和力ＡＣＴＨ受体的ＫＤ值为０．１５ｎｍｏｌ／Ｌ，Ｂｍａｘ为１．２ｆｍｏｌ／１×１０￣６细胞，１５８０位点／细胞，低亲和力受体ＫＤ值为６．１ｎｍｏｌ／Ｌ，Ｂｍａｘ为３７．５ｆｍｏｌ／１×１０￣６细胞，３２０００位点／细胞。胸腺细胞、Ｔ、Ｂ淋巴细胞及腹腔巨噬细胞（Ｍφ）亦具有高、低亲和力不同的ＡＣＴＨ受体。

It was reported that rat immune cells carry both high and low affinity ACTH receptor(ACTHR)·Splenocyte, its high affinity ACTHR had 366 sites/cell,its KD=0.11nmol/L and Bmax=1.2fmol/2 × 10￣6 cells and low affinity ACTHR had 42000 sites/cell,its KD=5.7nmol/L and Bmax=147fmol/2×10￣6 cells.Peripheral blood lympho-cyte(PBL),its high affinity ACTHR had 1580 sites/cell,its KD=0.15nmol/L and Bmax=1.2fmol/1×10￣6 cells,and low affinity ACTHR had 32000 sites/cell, its KD=6.1 nmol/L and Bmax = 37.5fmol/1×10￣6 cells. Thymocyte,its high affinity ACTHR had 840 sites/cell with KD=0.15nmol/L and Bmax= 2.8fmol/2×10￣6 cells, and low affinity ACTHR had 12400 sites/cell with KD=4.2nmol/Land Bmax = 41.5fmol/2×10￣6 cells,T lymphocyte separated by nylon wool column method, its high affinity ACTHR had 66 sites/cell with KD=0.1 nmol/L and Bmax=0.22 fmol/2×10￣6 cells and low affinity ACTHR had 2600 sites/cell with KD = 6.1 nmol/L and Bmax = 8.8 fmol/2 × 10￣6 cell.B lymphocyte separated by anti-θ-serum pluS complement,its high affinity ACTHR had 1470 sites/cell with KD=0.24nmol/L and Bmax=1.7fmol/7 × 10￣5 cells,and low affinityACTHR had 9976 sites/cell with KD=2.4 nmol/L and Bmax = 11.6 fmol/7×10￣5 cells. Peritoneal adherent cells( Mφ),its high affinity ACTHR had 516 sites/csll,with KD=0.08nmol/L,and Bmax= 0.86 fmol/1×10￣6 cells,and low affinity ACTHR had 22000 sites/cell,with KD =10.5nmol/Land Bmax= 36.8fmol/1×10￣6 cells.