去甲肾上腺素对大鼠腹腔巨噬细胞内IP_3、［Ca^（2＋）］_i和Ia抗原表达

Effect of norepinephrine on the IP_3 content,[Ca￣(2+)]i and Ia antigen of rat peritoneal macrophages

本文应用细胞膜放射标记和离子交换层析法测定巨噬细胞（ＭФ）内肌醇－１，４，５－三磷酸（ＩＰ３）、用Ｃａ￣（２＋）指示剂的分光光谱法测定ＭФ内Ｃａ￣（２＋）浓度（［Ｃａ￣（２＋）］）、用ＡＰＡＡＰ桥联酶标法检测ＭФ表面Ｉａ抗原的表达，研究去甲肾上腺素（ＮＥ）对大鼠腹腔的ＭФ内ＩＰ３、［Ｃａ￣（２＋）］ｉ和ＭФ表面ｌａ抗原表达的影响。结果显示ＮＥ（１０￣（－８）ｍｏｌ／Ｌ）可显著增高ＭФ中ＩＰ＿３含量（４４２±２２ｃｐｍ／１０￣６ｃｅｌｌｓ，对照组１０２±８ｃｐｍ／１０￣６ｃｅｌｌｓ，Ｐ＜０．０１）；ＮＥ可使ＭФ内［Ｃａ￣（２＋）］ｉ显著增高（３２２±７８ｎｍｏｌ／Ｌ，对照组９７±１７ｎｍｏｌ／Ｌ，Ｐ＜０．０１）；ＮＥ可显著增高ＭФ表面Ｉ－Ａ、Ｉ－Ｅ抗原的表达（６４±８％、５８±６％，对照组５０±３％，４４±４％，Ｐ＜０．０１）。提示神经递质ＮＥ促进ＭФ表面Ｉａ抗原表达的作用可能是通过第二信使ＩＰ＿３和Ｃａ￣（２＋）介导的。

The effect of norepinephrine(NE)on the inositol-1,4,5-trphosphate(IP_3)content,intracellular calcium ion concentration([Ca￣(2+)]_i)and Ia antigen cxpression of ratperitoneal macrophages(MФ)was studied. Cell membrane isotope lablling and ion-exch-ange chromatography were used to determine the IP_2 content,Fura-2 was used as afluorescent indicator to measure the[Ca￣(2+)]i and alkaline phosphatase anti-alkaline phos-phatase(APAAP)enzyme immunoassay was used to detect the expression of Ia antigenon the MФs. The results showed that NE (10￣(-8) mol/L)markedly increased the IP_3 content(NE group:442 ± 22 cpm/10￣6 cells,control:102 ±8 cpm/10￣6 cells,P<0.01) and[Ca￣(2+)]1(NE group: 322 ± 78 nmol/L,control:97 ±17nmol/L,P<0.01)of MФs. NEcould also promote the expression of I-A and I-E antigens on the MФs(NE group:64 ±8%and 58± 6%,control:50 ± 3%and 44 ± 4%,P<0.01).These findings suggestedthat the promotive effect of NE on the Ia a ntigen expression of the MФs was mediatedby the intracellular second messengers IP_3 and Ca￣(2+).