溶血与非溶血时血浆及红细胞过氧化氢诱发化学发光值比较

Comparison of H_2_O2 induced chemiluminescence between blood plasma and erythrocyte before and after hemolysis in rabbits

本实验观察了血细胞对血浆过氧化氢诱发化学发光值（Ｈ＿２Ｏ＿２－ＩＣＬ）的影响。结果表明，正常兔血浆Ｈ＿２Ｏ－２－ＩＣＬ值明显高于全血和红细胞悬液，发生溶血后上述三组份的发光值均显著增加，其中全血和血浆的发光值分别增加１５．５和６．１倍。油酸肺损伤兔所有组份的发光值均显著高于对照兔，同时其血浆Ｈ＿２Ｏ＿２－ＩＣＬ发光值的衰变系数明显低于对照兔。以上结果提示：红细胞及其碎片显著影响血浆Ｈ＿２Ｏ＿２－ＩＣＬ值，溶血促使其发光增强，而油酸肺损伤时血浆Ｈ＿２Ｏ＿２－ＩＣＬ值的显著增加，则提示氧自由基代谢产物是其发光值增加的物质基础。

he chemiluminescene induced by H_2O_2(H_2O_2-ICL)in arterial blood,blood plasma, and erythrocyte were measured before and after hemolysis in control andoleic acid(OA)induced lung injury rabbits,The results showed that the peak of H_2O_2-ICL(P-ICL)in blood plasma was highest in all samples and hemolysis will largelyenhance the H_2O_2-ICL of arterial blood and plasma,Compared with control,the P-ICLof all samples in OA were higher and the decay coefficient of ICL were smaller in OAthan in control. The results suggested that obviously hemolysis have an effect on H_2O_2-ICL in blood plasma and intact erythrocyte would inhibit the H_2O_2-ICL in plasma sig-nificantly. Oleic acid lung injury markedly increases the H_2O_2 -ICL because of oxidative Stress