脑组织钙、钙调素与兔感染性脑水肿关系的探讨

Relationship between calcium contents,calmodulin in brain tissue and infectious brain edema in rabbits

采用百日咳菌液右侧颈内动脉注射造成家兔急性感染性脑水肿（ＢＥ），测定不同时间脑组织水、钙、钙调素（ＣａＭ）含量，注射菌液后１ｈ，右侧脑组织水、钙含量均已明显增高（Ｐ＜０．０１），脑组织ＣａＭ含量也相应增高，注射菌液后２ｈ，其含量较对照组差异具有显著性意义（Ｐ＜０．０５），ＢＥ的发展与脑组织钙与ＣａＭ变化均呈正相关（ｒ＝０．８９０２，ｒ＝０．７９２５，Ｐ＜０．０１）。结果提示：脑组织钙聚积和ＣａＭ活性异常增高与感染性ＢＥ发病的关系密切。

The brain edema(BE) model was induced by injection of bordetella pertussissuspension( BPS)into right internal carotid artery of rabbits,inject normal saline as control.The contents of water,calcium and calmodulin(CaM)in brain tissue were measured atdifferent times after the injection of BPS.Average water and calcium contents in theright hemispheres at one hour after the injection of BPS were 81.40±1.12%and 1 0. 87±2.15 mmol/kg dry brain tissue respectively. They were all significantly higher thanthose of the controls(P<0.01).The CaM content in the right hemisphere at t wo hoursafter the injection were notably higher than that of the control(P<0.05).The contentsof water,calcium and CaM increased continuously as the BE developed.There was a goodlinear correlation between the contents of calcium, CaM and water in the right hemishphe-res during BE development(r= 0.8902,r=0.7925 P<0.01).These results suggested thatdevelopment of infectious BE are closely related to calcium accumulation and pathologicalincrease of CaM activity in the brain tissue.