硒和维生素E与内皮素在克山病心肌坏死发生中的作用

Effect of selenium and Vitamin E on the endothelin concentration and their roles in developing myo- cardial necrosis of Keshan disease

本研究以低硒和维生素Ｅ（ＶＥ）的克山病病区粮饲养大鼠，用放免法测定其血浆和心肌的内皮素（ＥＴ），发现克山病病区组ＥＴ水平明显高于其它各组；同时伴有谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活力下降，自由基净含量和脂质过氧化物（ＬＰＯ）浓度增高。ＥＴ升高和ＧＳＨ－Ｐｘ活力呈负相关，和自由基净含量、ＬＰＯ浓度呈正相关。结果提示：ＥＴ水平的变化可能作为一种中间环节参与克山病的发病机制；ＥＴ升高可能是使克山病的心肌坏死具有缺血缺氧特征的一个重要因素；硒和ＶＥ不仅可以改善心肌自由基代谢紊乱状态，而且很可能通过由自由基和ＬＰＯ或其它尚未知的环节影响ＥＴ的水平。

The concentration of plasma and myocardium endothelin(ET)in rats,which Were fed grains from Keshan disease( KD )endemic area, was determined by spec- ific radioimmunoassay. The results showed that the levels of ET of myocardium,freeradical( FR )and lipid peroxides(LPO )of plasma and myocardium in endemic groupwere significantly higher than those of the control,but glutathione peroxidase( GSH- Px )activity was reduced.The ET concentration was positively correlated with FR andLFO,and it was negatively correlated with GSH-Px activity. The results suggested thatalteration of ET concentration possible was a factor which plays a part in developing KDmyocardical necrosis.