鼠疫耶尔森菌新毒力决定因子

A NEW VIRULENT DETERMINANT OF YERSINIA PESTIS

以鼠疫耶尔森菌锡林郭勒高原型(简称鼠疫菌锡型)为研究模型,以鼠疫菌已知使人致病的生态型做对照,经多年的研究,于世界上首先提出32—40kd膜蛋白簇的有无及结构做为鼠疫耶尔森菌新毒力决定因子,它有别于已知的4个毒力决定因子,其对鼠疫菌毒力的决定作用已为流行病学、动物试验和志愿者试验所证实。

A new virulent determinant of Yersinia pestis was discovered by animal experiments, epizootiol-ogy,epidemiology,and volunteer experiment,in China which is 32-40 kd (kilodalton)protein group in all of known virulent strains of Y. pestis ecotype, high pathogenicity for human being, and the 32 - 40 kd proteins are located on OM (outer membrane ) of virulent strains of Y . pestis, in contrast to there is not 32 kd OMP in Y. pestis Xilingete plateau isolated from Microtus brandti, and the 40 kd protein is not located on OM of this ecotype which is similar to all of avirulent strains of Y. pestis. The results was proved by SDS-PAGE, Western blot, and McAb ELISA . It is suggested that though there 4 virulent determinants (Fra 1, Vwa, Pst1 and Pgm)in Y. pestis Xilingele plateau, it will not be harm to human being.