真菌毒素DON、T—2和NIV对培养软骨细胞作用的实验研究

THE EXPERIMENTAL STUDY OF EFFECT OF MYCOTOXIN DON, T-2 AND NIV ON THE CULTURAL CHONDROCYTES

采用软骨细胞体外单层培养方法,观察DON、T—2毒素和NIV对幼兔关节软骨细胞的作用。结果显示:三种毒素对培养的软骨细胞均有较强的毒性作用,能抑制DNA的合成和细胞的分裂增殖,特别是在细胞培养的早期,亦即在细胞分离时受到的损伤尚未完全恢复和细胞分裂增殖的旺盛时期,其损伤作用更为明显。三种毒素均对培养的软骨细胞膜系统具有明显的损伤作用,包括对细胞膜、核膜及所有细胞器的损害。实验结果对真菌毒素致软骨损害的机制提供了依据,也为KBD的病因及发病机制提供了重要线索。对真菌毒素与大骨节病病因间的关系进行了初步的讨论,但仍有一些问题须进一步研究探讨。

By using the method of chondrocytes in monolayer culture, we observed the effect of deoxyni-valenol (DON), T-2 toxin and nivalenol (NIV) on the rabbit articular chondrocytes. It was shown that the cultural chondrocytes were damaged strongly for all three kinds of toxin; that the synthesis of DNA and divided reproduction of chondrocytes were restrained; that the effect of damage was more evident, particularly at early stage of cultural chondrocytes, that is at the stage when damaged cells had not recovered from the cells separation and at the stage when chondrocytes divide and reproduce vigorously ; and that the membranes system including cell membrane, nucleus membrane and all subcellular organelles of the cultural chondrocytes was damaged strongly for all three kinds of toxin. The mechanism for the damage effect of mycotoxin on cartilage may be an important clue to etiopathogenesis of Kaschin-Beck disease. The relationship between mycotoxin and Kaschin-Beck disease was discussed preliminarily, but it still needs further investigation.