用原位核酸杂交法探讨柯萨奇B组病毒与克山病的关系──（黑龙江省急型克山病）

Relationship between Coxsackie Virus B and Acute Keshan disease by using nucleic acid hybridization in situ

为探讨柯萨奇Ｂ组病毒感染与黑龙江省急型克山病的关系。本文用缺口平移法制备生物标记的柯萨奇Ｂ３病毒的ｃＤＮＡ探针与１３例急型克山病尸检心肌组织及脑外伤、ＣＯ中毒等意外死亡的正常成人心肌组织１０例，非病区７～８个月引产胎儿心肌组织１０例的石腊切片进行原位杂交。结果１３例急型克山病中有８例出现阳性杂交信号，即有柯萨奇Ｂ组病毒的ＲＮＡ存在，阳性率达６１．５％。对照组均为阴性，提示黑龙江省急型克山病有柯萨奇Ｂ组病毒感染，可能是急型克山病发病因素之一。

We used hybridization in situ to examine RNA of Coxsackie Virus B(CVB) in paraffin-embedded myocardial specimens from 13 patients with acute Keshan disease and 10 patients with cerebral trauma or carbon monoxide poisoning and 10 foetus from non -endemic area. Of the 13 patients with acute Keshan disease, 8 (61. 5 % ) showed positive findings. Their myocardial tissues were infected by CVB previously. The results suggested that CVB infection may be one of the major cause of acute Keshan disease in Heilongjing province.