丙丁酚对氧化型低密度脂蛋白和氧自由基促血管平滑肌细胞增殖的影响

The Effect of Probucol on Multiplication of Vascular Smooth Muscle Cells Induced by Oxidized Low Density Lipoprotein and Oxygen Free Radicals

本文用细胞计数和ＭＴＴ快速比色计量法观察到体外培养的半主动脉平滑肌细胞分别与氧化型低密度脂蛋白和黄嘌呤－黄嘌呤氧化酶共厕培养后，前者明显增殖（Ｐ＜０．０１）。抗氧化剂丙丁酚（１００μｍｏｌ·Ｌ－１）能抑制氧化型低密度脂蛋白和黄嘌呤－黄嘌呤氧化酶促平滑肌细胞增殖作用；一氧化氮合成酶抑制剂ＮＧ－硝基－Ｌ－精氨酸对两者促平滑肌细胞增殖的作用无影响，同时也不能阻断丙丁酚的抑制两者促细胞增殖作用．这一结果提示，丙丁酚能抑制氧化型低密度脂蛋白和外源性氧自由基促平滑肌细胞增殖，这一作用可能与血管平滑肌细胞中一氧化氮的合成及释放无关。

By means of cell counting and MTT assay, we observed that cultured bovine aortic smooth muscle cells (SMC) incubated with oxidized low density lipoprotein (OLDL, 0. 2 g protein·L-1 )for 12 hours and xanthine (X, 100 μmol·L-1) plus xanthine oxidase(XO, 100 U. L-1 ) for 2 hours showed multiplication of bovine aortic smooth muscle cells and that the effects of OLDL and X-XO were significantly inhibited by probucol. Synthesis inhibitor of nitric oxide (NO) NG-nitro-L-arginine(NG-L-Arg, 100 μmol· L-1) could not abolish the effect of probucol. Our data indicates that probucol could contend with multiplication of SMC induced by OLDL or X-XO.